Expression analysis of the miRNA using stem-loop qRT-PCR To confirm the miRNA expression data, stem-loop qRT-PCR was performed as described in Chen et al. . Primers employed in the qRT-PCR are listed in S1 File. Genetics 77:521–534Google ScholarOka HI (1978) Phylogenetic differentiation of cultivated rice, XXI. The sprophytic pollen sterility: its genetic basis and intervarietal relationships as shown by F2 sterility. FunctIntegr Genomics. 2016; 16(3): 221–33. 46. Wei L, Zhang D, Xiang F, Zhang Z. Differentially expressed miRNAs potentially involved in the regulation of defense mechanism to drought stress in maize seedlings. IntroductionTo respond to changes in the environment, plants have developed remarkable capabilities to adapt to extreme environments, such as high temperature, drought, and salt stress . Abiotic stress is an important factor that limits plants geographic distribution and yield.
Plant Biotechnol J. 2015; 13(3): 293–305. pmid:25583362 52. Sharma N, Tripathi A, Sanan-Mishra N. Profiling the expression domains of a rice-specific microRNA under stress. Acta Genet Sinica 20:222–228Google ScholarZhang G, Lu Y, Zhang H, Yang J, Lin G (1994) Genetic studies of the hybrid sterility in cultivated rice (Oryza sativa L.). IV. Genotypes for F1 pollen sterility. How to make it work?? [Re] FPV App Do Not Workhi, can you take a photo that we can check it? Jpn J Genet 44:149–156Google ScholarShinjyo C (1972) Distributions of male sterility inducing cytoplasms and fertility restoring genes in rice, II. Varieties introduced from sixteen countries. Theor Appl Genet 84:720–724Google ScholarDellaporta SL, Wood J, Hicks JB (1984) Maize DNA miniprep. In: Russell M (ed) Molecular biology of plants. A laboratory course manual. Moreover, 88 miRNAs that were differentially-expressed were predicted to match with 197 targets for drought-stress.
Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 2005; 33(20): e179. pmid:16314309 35. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. See participating dealer for other restrictions and complete details. 10. Always use safety belts and child restraints. The miR395f and oru-miR47 were reduced in the root after drought stress, which was consistent with deep sequencing and indicated that the accuracy of the sequencing data was high. Furthermore, miR169 targets NF-YA2 to control root architecture . Recently, much research onmiRNAs function has been reported in cultivars rice.